Difference between revisions of "Part:BBa K2387010:Design"
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===Design Notes=== | ===Design Notes=== | ||
A long flexible linker was used to fuse CpxA and eYFPn to maximize the availability of eYFPn[1-154] and thus increase the chances to reassemble with eYFPc[155-238]. | A long flexible linker was used to fuse CpxA and eYFPn to maximize the availability of eYFPn[1-154] and thus increase the chances to reassemble with eYFPc[155-238]. | ||
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===Source=== | ===Source=== | ||
Revision as of 12:21, 16 October 2017
CpxA-eYFPn[1-154] fusion + araC/pBAD promoter
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
A long flexible linker was used to fuse CpxA and eYFPn to maximize the availability of eYFPn[1-154] and thus increase the chances to reassemble with eYFPc[155-238].
Source
eYFP originates from the jellyfish Aequorea victoria
araC/pBAD is an endogenous E. coli promoter
These original parts are taken from the iGEM repository; [http://parts.igem.com/BBa_E0030 eYFP] [http://parts.igem.com/BBa_I0030 araC/pBAD promoter] [http://parts.igem.com/BBa_B0034 RBS BBa_B0034] [http://parts.igem.com/Part:BBa_K1486004 Flexible Linker]
[http://parts.igem.com/Part:BBa_K2387002 CpxA] was amplified from the E. coli K12 genome during [http://2017.igem.org/Team:Wageningen_UR Wageningen_URs 2017 iGEM project].
eYFPc[1-154] was created in during [http://2017.igem.org/Team:Wageningen_UR Wageningen_URs 2017 iGEM project].