Difference between revisions of "Part:BBa K2269027"
 
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<partinfo>BBa_K2269027 short</partinfo>
 
<partinfo>BBa_K2269027 short</partinfo>
  
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Coding sequence corresponding to Flowering Locus T from Arabidopsis thaliana under the control of a chimeric promoter containing Etr8 (an E-responsive operator motif) fused to the minimal CMV promoter (Müller et al., 2014). FT has been optimized for Nicotiana benthamiana in order to promote floral induction in response to day length (Andres et al., 2015).  
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Transcriptional unit composed of the 35s inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB). In normal basis, a YFP will be expressing and only when RDF appears, luciferase will be expressed.  
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 10:06, 15 October 2017


Tnos:YFP:Assembly Reporter PB:Luc:Tnos

Transcriptional unit composed of the 35s inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB). In normal basis, a YFP will be expressing and only when RDF appears, luciferase will be expressed.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 819
    Illegal XbaI site found at 506
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 819
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 819
    Illegal BglII site found at 302
    Illegal BglII site found at 542
    Illegal BglII site found at 649
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 819
    Illegal XbaI site found at 506
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 819
    Illegal XbaI site found at 506
  • 1000
    COMPATIBLE WITH RFC[1000]