Difference between revisions of "Part:BBa K2269010"

 
 
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<partinfo>BBa_K2269010 short</partinfo>
 
<partinfo>BBa_K2269010 short</partinfo>
  
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Module for the regulated expression of firefly luciferase driven by the synthetic promoter Etr8-minCMV and the constitutive expression of chimeric constructs of A. thaliana PhyB-VP16-NLS and PIF6-NLS.  
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Middle part for assembly of site-specific recombination based Registers. Composed of the 35s inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attR).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 10:47, 14 October 2017


Register Assembly 2/3 PB

Middle part for assembly of site-specific recombination based Registers. Composed of the 35s inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attR).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1461
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1461
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1461
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1461
  • 1000
    COMPATIBLE WITH RFC[1000]