Difference between revisions of "Part:BBa K2368009:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Firstly , we construct the specific primer that consists of the gene sequence of HIS tag. Then， we combine T1R2 with the HIS by the method of PCR. | |
| − | + | ||
===Source=== | ===Source=== | ||
===References=== | ===References=== | ||
Revision as of 03:01, 14 October 2017
His+T1R2 overlap
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Firstly , we construct the specific primer that consists of the gene sequence of HIS tag. Then， we combine T1R2 with the HIS by the method of PCR.