Difference between revisions of "Part:BBa K2368009"
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<partinfo>BBa_K2368009 short</partinfo>
 
<partinfo>BBa_K2368009 short</partinfo>
  
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In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct T1R2-6*His tag. This tag can be recognized by specific monoclonal antibodies then we use fluorescence second antibody to interact with monoclonal antibodies to show the T1R2’s expression and location.  
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<P>This part is that sweetness receptor T1R2 combines with the HIS tag. </P>
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Because the T1R2 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein αsubunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R2 and His tag. And hope to show the location of T1R2 through immunofluorescence for detecting His tag.
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<P>The T1R2 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway is so important that it can’t be modified. Also, it is necessary for us to show whether the T1R2 expressed correctly. As a result of that, we can only perform the fusion between the N-terminal of T1R2 and HIS tag.</P>
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<P>The result of the part is to detect the expression and the location of the T1R2.</P>
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Revision as of 02:59, 14 October 2017


Introduction

His+T1R2 overlap

This part is that sweetness receptor T1R2 combines with the HIS tag.

The T1R2 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway is so important that it can’t be modified. Also, it is necessary for us to show whether the T1R2 expressed correctly. As a result of that, we can only perform the fusion between the N-terminal of T1R2 and HIS tag.

The result of the part is to detect the expression and the location of the T1R2.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]