Difference between revisions of "Part:BBa K2368008:Design"
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
This is a complete protein expression box of T1R3.
+
We construct the part by Gibson assembly.And the Gal1/Gal10 promoter and the ADH1 terminator stem from the PESC-Ura plasmid.
−
 
+
 
===Source===
 
===Source===
  

Revision as of 02:52, 14 October 2017


PGal 1/10-T1R3-ADH1t


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 563
    Illegal BglII site found at 2466
    Illegal BglII site found at 2490
    Illegal BglII site found at 2991
    Illegal BglII site found at 3072
    Illegal BamHI site found at 674
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 291
    Illegal AgeI site found at 747
    Illegal AgeI site found at 1149
    Illegal AgeI site found at 2751
    Illegal AgeI site found at 3351
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2455
    Illegal BsaI.rc site found at 3185


Design Notes

We construct the part by Gibson assembly.And the Gal1/Gal10 promoter and the ADH1 terminator stem from the PESC-Ura plasmid.

Source

References