Difference between revisions of "Help:Submission"

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{{HelpPage/MainLinks}}
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#redirect [[DNA_Submission#Submission_Requirements]]
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<div id="splash-title">Submission Requirements</div>
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<p>You've <a href="https://parts.igem.org/Help:Adding_Parts">added your part</a> to the Registry, but before you <a href="https://parts.igem.org/cgi/dna_transfer/index.cgi">send a DNA sample</a> to the Registry, here's how you prepare your parts for the Registry. Remember, the Registry is built on standard parts!</p>
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<p>If you have questions or issues concerning the requirements, contact <b>hq (at) igem (dot) org</b>.</p>
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<div class="link_box" style="bottom: 15px;"> <a href="https://parts.igem.org/cgi/dna_transfer/index.cgi">
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    <div id="sendapart" class="link_image"></div>
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    <div class="link_text" style="line-height: 20px;">know what to do? send your sample now!</div>
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<h3><img src="https://static.igem.org/mediawiki/parts/5/58/List-gear-1.png" style="width: 30px; height: 30px;" /> <a href="https://parts.igem.org/Part:pSB1C3">pSB1C3</a></h3>
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<ul><b>Parts must be in pSB1C3, <a href="https://parts.igem.org/Help:Shipping">the Registry's standard shipping backbone</a></b>
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<li>Your part will be flanked by the <a href="https://parts.igem.org/Help:Prefix-Suffix">BioBrick prefix and suffix</a> on the backbone</li>
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<li>The Registry can test and maintain all parts in the same way</li>
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<li>pSB1C3 is a high-copy plasmid, which improves miniprep yields for parts. (If your part is toxic at high copy, use <a href="https://parts.igem.org/Part:pSB3C5">pSB3C5</a>)</li>
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<li>Users can easily move/assemble the part into <a href="https://parts.igem.org/Help:Plasmid_Backbones">another plasmid backbone</a></li>
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<li>You can use the pSB1C3 <a href="https://parts.igem.org/Help:Protocols/Linearized_Plasmid_Backbones">linearized plasmid backbone</a> (in kit or by request) or the pSB1C3 samples in the distribution kit.</li>
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<h3><img src="https://static.igem.org/mediawiki/parts/d/d0/List-gear-2.png" style="width: 30px; height: 30px;" /> <a href="https://parts.igem.org/Help:Prefix-Suffix">BioBrick compatible</a></h3>
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<ul><b>Parts must be <a href="https://parts.igem.org/Help:Assembly_Compatibility">BioBrick (RFC 10) compatible</a>
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</b>
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<li>Your part's sequence must not contain the restriction sites of the <a href="https://parts.igem.org/Help:Prefix-Suffix">BioBrick prefix and suffix</a>: <b>EcoRI, XbaI, SpeI, PstI</b>.</li>
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<li>The Registry can test and maintain all parts in the same way</li>
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<li>Your part can be assembled to others with 3A Assembly.</li>
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<li>Users can easily move/assemble the part into <a href="https://parts.igem.org/Help:Plasmid_Backbones">another plasmid backbone</a></li>
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<li>Check if your part is BioBrick compatible by looking at the part's <a href="https://parts.igem.org/Help:Sequence_Features">sequence and feature box</a>. </li>
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<h3><img src="https://static.igem.org/mediawiki/parts/d/dd/List-gear-3.png" style="width: 30px; height: 30px;" /> <a href="https://parts.igem.org/Add_a_Part_to_the_Registry">Documented</a></h3>
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<ul><b>Parts shipped to the Registry must be documented on the Registry.</b>
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<li>You must <a href="https://parts.igem.org/Add_a_Part_to_the_Registry">add your part</a> to the Registry; it will then receive a part name (BBa_...)</li>
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<li>Your part's <b>sequence</b> must be entered into the Registry</li>
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<li><b>Quality over quantity:</b> sending a few well-documented parts is always better than sending several that are not</li>
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<li>For examples check out the <a href="https://parts.igem.org/Well_Documented_Parts">collection of well documented parts.</li>
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<h3><img src="https://static.igem.org/mediawiki/parts/1/11/List-gear-4.png" style="width: 30px; height: 30px;" /> <a href="http://2014.igem.org/Safety">Safety</a></h3>
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<ul><b>iGEM teams must meet the standards set by the <a href="http://2014.igem.org/Safety">safety committee</a></b>
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<li>The Registry accepts parts from Risk Group 1 organisms.</li>
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<li>The Registry may accept parts from Risk Group 2 and 3 organisms that have received authorization from the Safety committee</li>
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<li>Please see the <a href="http://2014.igem.org/Safety/White_List">Safety Hub's White List</a> for more detailed information</li>
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<div id="splash-title">Submission Form</div>
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<ul>Now that your part meets the Registry's submission requirements, you're ready to <a href="https://parts.igem.org/cgi/dna_transfer/index.cgi">send a DNA sample</a>:
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<li>you'll need to fill out an online submission form</li>
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<li><a href="https://parts.igem.org/Help:Submission_Form/Formats">prepare the physical shipment</a> (aliquot DNA into tubes or wells). Avoid leaky tubes! Choose a Registry accepted format!</li>
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<li>ship the DNA to iGEM HQ (include a tracking number)</li>
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</ul>
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<br>
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<p><span style="color: red; font-weight: bold;">Note to iGEM Teams:</span> Your BioBrick Part DNA is <b>due at</b> iGEM HQ by the deadline on the <a href="">Calendar of Events</a>. Make sure to ship it before the deadline, allowing for transit time.</p>
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<a href="https://parts.igem.org/Help:Submission/Form" class="navbutton" style="float: right;">submission form instructions >></a>
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Latest revision as of 17:20, 13 October 2017