Difference between revisions of "Help:Submission"

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#redirect [[DNA_Submission#Submission_Requirements]]
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<a href="https://parts.igem.org/Help:Submission_Form" class="navbutton" style="float: right;">submission form instructions >></a>
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<div id="splash-title">Sending Parts</div>
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<ul><b>Parts submitted to the Registry must be:</b>
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<li>in pSB1C3</li>
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<li>BioBrick compatible</li>
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<li>documented on the Registry</li>
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<li>safe!</li>
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<p>You've <a href="https://parts.igem.org/Help:Adding_Parts">added your part</a> to the Registry, but before you <a href="https://parts.igem.org/cgi/dna_transfer/index.cgi">send a DNA sample</a> to the Registry, make sure that your part follows the Registry's submission requirements and instructions.</p>
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<p>If you have questions or issues concerning the requirements, contact <b>hq (at) igem (dot) org</b>.</p>
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<div id="splash-title">Submission Requirements</div>
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<h3><img src="https://static.igem.org/mediawiki/parts/5/58/List-gear-1.png" style="width: 30px; height: 30px;" /> <a href="https://parts.igem.org/Part:pSB1C3">pSB1C3</a></h3>
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<ul><b>Parts must be in pSB1C3, <a href="https://parts.igem.org/Help:Shipping">the Registry's standard shipping backbone</a></b>
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<li>Your part will be flanked by the BioBrick prefix and suffix on the backbone</li>
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<li>The Registry can test and maintain all parts in the same way</li>
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<li>pSB1C3 is a high-copy plasmid, which improves miniprep yields for parts</li>
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<li>Users can easily and reliably move or assemble the part into another plasmid backbone</li>
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<h3><img src="https://static.igem.org/mediawiki/parts/d/d0/List-gear-2.png" style="width: 30px; height: 30px;" /> <a href="https://parts.igem.org/Help:Prefix-Suffix">BioBrick compatible</a></h3>
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<ul><b>Parts must be BioBrick (RFC 10) compatible</b>
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<li>Your part's sequence must not contain the restriction sites of the <a href="https://parts.igem.org/Help:Prefix-Suffix">BioBrick prefix and suffix</a>: <b>EcoRI, XbaI, SpeI, PstI</b>.</li>
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<li>The Registry can test and maintain all parts in the same way</li>
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<li>Your part can be assembled to others with 3A or Standard Assembly.</li>
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<li>Check if your part is BioBrick compatible by looking at the part's <a href="https://parts.igem.org/Help:Adding_Parts#Sequence_and_Features">sequence and feature box</a>. </li>
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<h3><img src="https://static.igem.org/mediawiki/parts/d/dd/List-gear-3.png" style="width: 30px; height: 30px;" /> <a href="https://parts.igem.org/Add_a_Part_to_the_Registry">Documented</a></h3>
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<ul><b>Parts shipped to the Registry must be documented on the Registry.</b>
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<li>You must <a href="https://parts.igem.org/Add_a_Part_to_the_Registry">add your part</a> to the Registry; it will then receive a part name (BBa_...)</li>
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<li>Your part's <b>sequence</b> must be entered into the Registry</li>
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<li><b>Quality over quantity:</b> sending a few well-documented parts is always better than sending several that are not</li>
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<li>For examples of well documented parts check out: <a href="https://parts.igem.org/Part:BBa_K274210">BBa_K274210</a>, <a href="https://parts.igem.org/Part:BBa_F2620">BBa_F2620</a>, and <a href="https://parts.igem.org/Part:BBa_E0040">BBa_E0040</a></li>
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<h3><img src="https://static.igem.org/mediawiki/parts/1/11/List-gear-4.png" style="width: 30px; height: 30px;" /> <a href="http://2013.igem.org/Safety">Safety</a></h3>
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<ul><b>iGEM teams must meet the <a href="http://2013.igem.org/Safety">safety requirements</a></b>
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<li>The Registry accepts parts from Risk Group 1 organisms.</li>
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<li>The Registry may accept parts from Risk Group 2 organisms that have received authorization from the Safety committee</li>
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<li>The Registry will not accept any parts from Risk Group 3 and 4 organisms</li>
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<li>The Registry will not accept any parts from organisms or viruses on <a href="http://www.australiagroup.net/en/biological_agents.html">The Australia Group's List of Biological Agents for Export Control</a>.</li>
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<div id="splash-title">Submission Form</div>
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<ul>Now that your part meets the Registry's submission requirements, you're ready to <a href="https://parts.igem.org/cgi/dna_transfer/index.cgi">send a DNA sample</a>:
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<li>you'll need to fill out an online submission form</li>
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<li>prepare the physical shipment (aliquot DNA into tubes or wells)</li>
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<li>ship the DNA to iGEM HQ (include a tracking number)</li>
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<p><span style="color: red; font-weight: bold;">Note to iGEM Teams:</span> Your BioBrick Part DNA is <b>due at</b> iGEM HQ by the deadline on the <a href="http://2013.igem.org/Calendar_of_Events">Calendar of Events</a>. Make sure to ship it before the deadline, allowing for transit time.</p>
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<a href="https://parts.igem.org/Help:Submission_Form" class="navbutton" style="float: right;">submission form instructions >></a>
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<div id="splash-title">Helpful Resources</div>
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<li><a href="https://parts.igem.org/Help:Adding_Parts">Add your part</a> to the Registry</li>
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<li><a href="https://parts.igem.org/Help:Protocol/PCR_Standardization">BioBrick a part</a>: add the BioBirck prefix and suffix to your part through PCR</li>
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<li><a href="https://parts.igem.org/Help:Protocols/Linearized_Plasmid_Backbones">Linearized Plasmid Backbones</a>: transfer your part into the pSB1C3 linearized plasmid backbone</li>
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<li>Use site mutagenesis to remove illegal restriction sites</li>
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Latest revision as of 17:20, 13 October 2017

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