Difference between revisions of "Part:BBa K2197300"
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<partinfo>BBa_K2197300 short</partinfo> | <partinfo>BBa_K2197300 short</partinfo> | ||
| − | Engineered e.coli encodes part BBa_K2197300. By adding blood samples to culture medium which engineered e.coli is cultured, e.coli expresses different level of GFP. By analysing the GFP's fluorescence level with a plate reader , the concentration of uric acid can be determined This part ensures a rapid detection of uric acid concentration thus gout. | + | Engineered e.coli encodes part BBa_K2197300. By adding blood samples to culture medium which engineered e.coli is cultured, e.coli expresses different level of GFP. By analysing the GFP's fluorescence level with a plate reader, the concentration of uric acid can be determined. This part ensures a rapid detection of uric acid concentration thus gout. |
Revision as of 12:55, 13 October 2017
Expression of GFP under the control of a uric acid concentration-sensitive promoter device
Engineered e.coli encodes part BBa_K2197300. By adding blood samples to culture medium which engineered e.coli is cultured, e.coli expresses different level of GFP. By analysing the GFP's fluorescence level with a plate reader, the concentration of uric acid can be determined. This part ensures a rapid detection of uric acid concentration thus gout.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1098
Illegal NheI site found at 1121 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 555
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 291
Illegal BsaI.rc site found at 1814
Illegal SapI.rc site found at 255