Difference between revisions of "Part:BBa K2242005"

 
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__NOTOC__
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<partinfo>BBa_K2242005 short</partinfo>
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KMADH is an alcohol dehydrogenase from Kluyveromyces marxianus. It can catalyze the ethanol fermentation ongoing in the bacteria with a high efficiency and mild reaction condition, especially temperature.
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We use T7 promoter and Lac operator to control KMADH’s expression. Both of this two units are induced by IPTG. With this dual switch, we can reduce the leak of the gene expression as much as possible.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2242005 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2242005 parameters</partinfo>
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Revision as of 11:18, 12 October 2017


T7+lacO+KmAdh

KMADH is an alcohol dehydrogenase from Kluyveromyces marxianus. It can catalyze the ethanol fermentation ongoing in the bacteria with a high efficiency and mild reaction condition, especially temperature. We use T7 promoter and Lac operator to control KMADH’s expression. Both of this two units are induced by IPTG. With this dual switch, we can reduce the leak of the gene expression as much as possible.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 860
    Illegal XhoI site found at 922
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]