Difference between revisions of "Part:BBa K2447000"

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	<partinfo>BBa_K2447000 short</partinfo>		<partinfo>BBa_K2447000 short</partinfo>
−	PhoB and PhoR proteins are part of the Pho regulon inherent in E.coli. When low concentration of extracellular phosphate ions is present, PhoR can phosphorylate PhoB to form active phosphorylated-PhoB. PhoB promoter is activated as a result of active binding of phosphorylated-PhoB, resulting in downstream expression of GFP. When high concentration of extracellular phosphate ions is present, PhoR will dephosphorylate phosphorylated-PhoB, and therefore inactivating it, and repressing PhoB promoter for downstream expression of GFP.	+	PhoB and PhoR proteins are part of the Pho regulon inherent in E.coli. When low concentration of extracellular phosphate ions is present, PhoR can phosphorylate PhoB to form active phosphorylated-PhoB. PhoB promoter is activated as a result of active binding of phosphorylated-PhoB, resulting in downstream expression of GFP. When high concentration of extracellular phosphate ions is present, PhoR will dephosphorylate phosphorylated-PhoB, and therefore inactivating it, and repressing PhoB promoter for downstream expression of GFP. [[Image:Phosphate1.png|thumb|center|500px|PhoR and PhoB proteins work in tandem to control promoter PhoB and consequential downstream expression of GFP]]
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		+	====Improvement over previous iGEM part BBa_K116404 (NYMUTaipei 2008)====
		+	The fluorescence of purified GFPmut3B was calibrated in the [[Chassis/Cell-Free_Systems|cell-free chassis]]. The derived [http://2007.igem.org/Imperial/Wet_Lab/Results/Res1.3 calilbration curve]allows the determination of the concentration of GFPmut3b in the cell-free chassis. [http://2007.igem.org/Imperial/Wet_Lab/Protocols/Prot1.3 Detailed protocols]for generating the calibration curve are available. Other calibration curves for are also available on the results page.[[Image:Phosphate2.png|thumb|center|800px|PhoR and PhoB proteins work in tandem to control promoter PhoB and consequential downstream expression of GFP]]
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		+	[[Image:Phosphate3.png|thumb|center|800px|PhoR and PhoB proteins work in tandem to control promoter PhoB and consequential downstream expression of GFP]]
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−	<img src="https://smcl.org/blogs/post/2017-mid-autumn-festival/ ">
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Revision as of 00:29, 12 October 2017

Extracellular phosphate sensor with GFP reporter

PhoB and PhoR proteins are part of the Pho regulon inherent in E.coli. When low concentration of extracellular phosphate ions is present, PhoR can phosphorylate PhoB to form active phosphorylated-PhoB. PhoB promoter is activated as a result of active binding of phosphorylated-PhoB, resulting in downstream expression of GFP. When high concentration of extracellular phosphate ions is present, PhoR will dephosphorylate phosphorylated-PhoB, and therefore inactivating it, and repressing PhoB promoter for downstream expression of GFP.

Improvement over previous iGEM part BBa_K116404 (NYMUTaipei 2008)

The fluorescence of purified GFPmut3B was calibrated in the cell-free chassis. The derived [http://2007.igem.org/Imperial/Wet_Lab/Results/Res1.3 calilbration curve]allows the determination of the concentration of GFPmut3b in the cell-free chassis. [http://2007.igem.org/Imperial/Wet_Lab/Protocols/Prot1.3 Detailed protocols]for generating the calibration curve are available. Other calibration curves for are also available on the results page.

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Sequence and Features