Difference between revisions of "Part:BBa K2206004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | No terminators are needed as the pSB1C3 plasmid contains terminators. | |
| − | + | ||
| + | There is a scar between the promoter and the toehold switch, but there is no scar between the toehold switch and the reporter protein. | ||
===Source=== | ===Source=== | ||
Latest revision as of 13:32, 8 October 2017
Toehold switch for hsa-miR-15b-5p with luciferase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 928
Design Notes
No terminators are needed as the pSB1C3 plasmid contains terminators.
There is a scar between the promoter and the toehold switch, but there is no scar between the toehold switch and the reporter protein.
Source
This part is a synthetic toehold switch that was designed in silico and synthesised by IDT