Difference between revisions of "Part:BBa K2406053:Experience"
JackSuitor (Talk | contribs) |
JackSuitor (Talk | contribs) |
||
| Line 20: | Line 20: | ||
<!-- DON'T DELETE --><partinfo>BBa_K2406053 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K2406053 EndReviews</partinfo> | ||
Construct is shown to work through experiment where cells were treated with IPTG and RFP fluorescence was measured. Control cells were not treated with IPTG. Results clearly show RFP is only expressed in presence of IPTG and thus when Vika recombinase is expressed. This confirms function of Vika recombinase, T7-LacO promoter, and Vox target sites. | Construct is shown to work through experiment where cells were treated with IPTG and RFP fluorescence was measured. Control cells were not treated with IPTG. Results clearly show RFP is only expressed in presence of IPTG and thus when Vika recombinase is expressed. This confirms function of Vika recombinase, T7-LacO promoter, and Vox target sites. | ||
| − | [[BBa_K2406053.png|200px|thumb|left|Graph showing expression of RFP when cells with measurement construct are induced with IPTG compared to uninduced cells.]] | + | [[Image: BBa_K2406053-graph_1.png|200px|thumb|left|Graph showing expression of RFP when cells with measurement construct are induced with IPTG compared to uninduced cells.]] |
Latest revision as of 12:08, 7 October 2017
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2406053
This part is used to assay activity of Vika recombinase, whose expression is induced by treating cells with IPTG.
User Reviews
UNIQc889987e041b2bb2-partinfo-00000000-QINU UNIQc889987e041b2bb2-partinfo-00000001-QINU Construct is shown to work through experiment where cells were treated with IPTG and RFP fluorescence was measured. Control cells were not treated with IPTG. Results clearly show RFP is only expressed in presence of IPTG and thus when Vika recombinase is expressed. This confirms function of Vika recombinase, T7-LacO promoter, and Vox target sites.
