Difference between revisions of "Part:BBa K2404004"

Revision as of 11:54, 6 October 2017

GST6 shortened - a salicylic acid-induced promoter

The GST6 promotor is a regulatory sequence from Arabidopsis thaliana that has been shown to respond to Salicylic acid.

This promotor includes the sequence -407 > +90 in relation to the GST6 translational start point.

This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.

This part is has been cloned into the pSB1C3 plasmid using BsmBI.

The sequence includes an internal BsmBI site so was cloned into pSB1C3 by partial digestion. This can be used for generation of level 1 plasmids but not for subsequent generation of level 2 plasmids.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 540
Illegal PstI site found at 582
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 540
Illegal PstI site found at 582
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 607
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 540
Illegal PstI site found at 582
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 540
Illegal PstI site found at 582
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 794

@@ Line 9: / Line 9: @@
 This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.
 This part is has been cloned into the pSB1C3 plasmid using BsmBI.
+The sequence includes an internal BsmBI site so was cloned into pSB1C3 by partial digestion. This can be used for generation of level 1 plasmids but not for subsequent generation of level 2 plasmids.