Difference between revisions of "Part:BBa K2404004"

Revision as of 11:52, 6 October 2017

GST6 shortened - a salicylic acid-induced promoter

The GST6 promotor is a regulatory sequence from Arabidopsis thaliana that has been shown to respond to Salicylic acid.

This promotor includes the sequence -407 > +90 in relation to the GST6 translational start point.

This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.

This part is has been cloned into the pSB1C3 plasmid using BsmBI.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 540
Illegal PstI site found at 582
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 540
Illegal PstI site found at 582
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 607
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 540
Illegal PstI site found at 582
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 540
Illegal PstI site found at 582
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 794

@@ Line 5: / Line 5: @@
 The [https://apps.araport.org/thalemine/report.do?id=1010222 GST6 promotor] is a regulatory sequence from <i>Arabidopsis thaliana</i> that has been shown to respond to Salicylic acid.
-This promotor includes the sequence -407 > +90 from the GST6 translational start point.
+This promotor includes the sequence -407 > +90 in relation to the GST6 translational start point.
 This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.