Difference between revisions of "Part:BBa K2404004:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | - We isolated genomic DNA from wildtype Arabidopsis thaliana | |
+ | - We designed primers to amplify the GST6 promotor sequence.<br> | ||
+ | iGEM49 GST6pF tttCGTCTCtCTCAggagGGAAAGATGAGATTCGTTTC | ||
+ | <br>iGEM50 GST6pR tttCGTCTCtCTCGcattGGAATTGGATGAAGAAGAATG | ||
+ | |||
+ | - We gel extracted the DNA and digested with BsmBI to introduce the plasmid into pSB1C3. | ||
+ | |||
+ | - This plasmid corresponds to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard. | ||
Latest revision as of 11:45, 6 October 2017
GST6 shortened - a salicylic acid-induced promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 68
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 68
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 68
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 514
Design Notes
- We isolated genomic DNA from wildtype Arabidopsis thaliana
- We designed primers to amplify the GST6 promotor sequence.
iGEM49 GST6pF tttCGTCTCtCTCAggagGGAAAGATGAGATTCGTTTC
iGEM50 GST6pR tttCGTCTCtCTCGcattGGAATTGGATGAAGAAGAATG
- We gel extracted the DNA and digested with BsmBI to introduce the plasmid into pSB1C3.
- This plasmid corresponds to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard.
Source
This part comes from Arabidopsis thaliana and was isolated using specific primers.