Difference between revisions of "Part:BBa K2443043:Design"

 
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===Source===
 
===Source===
  
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Sequence obtained from:
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<p>Sequence obtained from:
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http://www.uniprot.org/uniprot/P21889
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http://www.uniprot.org/uniprot/P21889</p>
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His tag terminal:
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<p>C-terminal His tag chosen because of the following publication:
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Wang H., Huang P., Xu G., Haas W., Marblestone A., Li J., Gygi S., Forster A., Jewett M.,  Church G. (2012) Multiplexed in Vivo His- Tagging of Enzyme Pathways for in Vitro Single-pot Multienzyme Catalysis. <i>ACS Synth. Biol. 2</i>: 43-52
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Wang H., Huang P., Xu G., Haas W., Marblestone A., Li J., Gygi S., Forster A., Jewett M.,  Church G. (2012) Multiplexed in Vivo His- Tagging of Enzyme Pathways for in Vitro Single-pot Multienzyme Catalysis. <i>ACS Synth. Biol. 2</i>: 43-52</p>
  
 
===References===
 
===References===

Latest revision as of 22:27, 4 October 2017


Asp RS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 703


Design Notes

Codon optimized for use in Escherichia coli.



Source

Sequence obtained from: http://www.uniprot.org/uniprot/P21889

C-terminal His tag chosen because of the following publication: Wang H., Huang P., Xu G., Haas W., Marblestone A., Li J., Gygi S., Forster A., Jewett M., Church G. (2012) Multiplexed in Vivo His- Tagging of Enzyme Pathways for in Vitro Single-pot Multienzyme Catalysis. ACS Synth. Biol. 2: 43-52

References