Difference between revisions of "Part:BBa P10500"
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This sequence is contained within the BioBricks vector pSB1C3.
 
This sequence is contained within the BioBricks vector pSB1C3.
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[[File:BBa_P10500_Cloning_Instructions.png|700px]]
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[[File:BBa_P10500_Cloning_Instructions.pdf]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:08, 4 October 2017


PhytoBricks Universal Acceptor

This is the universal acceptor plasmid for creating new PhytoBricks parts. It contains the lacZ-alpha fragment in the cloning site for blue-white screening through the use of IPTG and X-Gal on the agar plates.

This sequence is contained within the BioBricks vector pSB1C3.


BBa P10500 Cloning Instructions.png

File:BBa P10500 Cloning Instructions.pdf


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 247
    Illegal XbaI site found at 220
    Illegal PstI site found at 208
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 247
    Illegal PstI site found at 208
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 247
    Illegal BamHI site found at 226
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 247
    Illegal XbaI site found at 220
    Illegal PstI site found at 208
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 247
    Illegal XbaI site found at 220
    Illegal PstI site found at 208
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 598