Difference between revisions of "Part:BBa K2368023"

 
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__NOTOC__
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<h1>Introduction</h1>
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<partinfo>BBa_K2368023 short</partinfo>
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<p>In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct T1R2-Myc tag. This tag can be recognized by specific monoclonal antibodies then we use fluorescence second antibody to interact with monoclonal antibodies to show the T1R2’s expression and location. </p>
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<p>Because the T1R2 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein αsubunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R2 and Myc tag. And hope to show the location of T1R2 through immunofluorescence for detecting Myc tag.</p>
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===Usage and Biology===
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<h2>Sequence and Features</h2>
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<partinfo>BBa_K2368023 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K23680232 parameters</partinfo>
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Revision as of 03:55, 4 October 2017


Introduction

Myc+T1R2 overlap

In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct T1R2-Myc tag. This tag can be recognized by specific monoclonal antibodies then we use fluorescence second antibody to interact with monoclonal antibodies to show the T1R2’s expression and location.

Because the T1R2 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein αsubunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R2 and Myc tag. And hope to show the location of T1R2 through immunofluorescence for detecting Myc tag.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]