Difference between revisions of "Part:BBa K2206001"

Revision as of 20:58, 2 October 2017

Toehold switch for hsa-miR-27b-3p

Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that activates translation of the downstream protein coding region.

This part codes for a toehold switch that contains a region that is complementary to the micro-RNA hsa-mir-27b-3p (the trigger RNA). In the presence of hsa-mir-27b-3p, translation of the downstream protein coding region occurs.

This part can be used to regulate the expression of any protein in response to the levels of hsa-mir-27b-3p present in a system.

We believe that the transcripts produced by this part are toxic when expressed at reasonably high concentrations in E.coli (e.g. the concentrations produced from the BBa_J23111 constitutive promoter).

N.B. This part contains a strong RBS sequence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2206001 short</partinfo>
-Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, resulting in translation of the protein coding region.
+Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that activates translation of the downstream protein coding region.
-This part codes for a toehold switch that contains a region that is complementary to the micro-RNA hsa-mir-27b-3p. In the presence of hsa-mir-27b-3p, translation of the downstream protein coding region occurs.
-This part can be used to regulate the expression of any protein in response to the presence of hsa-mir-27b-3p. This part contains a strong RBS. We believe that the transcripts produced by this part are toxic when expressed at reasonably high concentrations in E.coli (e.g. the concentrations produced from the BBa_J23111 constitutive promoter).
+This part codes for a toehold switch that contains a region that is complementary to the micro-RNA hsa-mir-27b-3p (the trigger RNA). In the presence of hsa-mir-27b-3p, translation of the downstream protein coding region occurs.
+This part can be used to regulate the expression of any protein in response to the levels of hsa-mir-27b-3p present in a system.
+We believe that the transcripts produced by this part are toxic when expressed at reasonably high concentrations in E.coli (e.g. the concentrations produced from the BBa_J23111 constitutive promoter).
+N.B. This part contains a strong RBS sequence.
 <!-- Add more about the biology of this part here