Difference between revisions of "Part:BBa K2206001"
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Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, resulting in translation of the protein coding region. | Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, resulting in translation of the protein coding region. | ||
This part codes for a toehold switch that contains a region that is complementary to the micro-RNA hsa-mir-27b-3p. In the presence of hsa-mir-27b-3p, translation of the downstream protein coding region occurs. | This part codes for a toehold switch that contains a region that is complementary to the micro-RNA hsa-mir-27b-3p. In the presence of hsa-mir-27b-3p, translation of the downstream protein coding region occurs. | ||
| − | This part can be used to regulate the expression of any protein in response to the presence of hsa-mir-27b-3p. This part contains a strong RBS. We believe that the transcripts produced by this part are toxic when expressed at reasonably high concentrations (e.g. the concentrations produced from the BBa_J23111 constitutive promoter). | + | This part can be used to regulate the expression of any protein in response to the presence of hsa-mir-27b-3p. This part contains a strong RBS. We believe that the transcripts produced by this part are toxic when expressed at reasonably high concentrations in E.coli (e.g. the concentrations produced from the BBa_J23111 constitutive promoter). |
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Revision as of 20:26, 2 October 2017
Toehold switch for hsa-miR-27b-3p
Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, resulting in translation of the protein coding region. This part codes for a toehold switch that contains a region that is complementary to the micro-RNA hsa-mir-27b-3p. In the presence of hsa-mir-27b-3p, translation of the downstream protein coding region occurs. This part can be used to regulate the expression of any protein in response to the presence of hsa-mir-27b-3p. This part contains a strong RBS. We believe that the transcripts produced by this part are toxic when expressed at reasonably high concentrations in E.coli (e.g. the concentrations produced from the BBa_J23111 constitutive promoter).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]