Difference between revisions of "Part:BBa K2368017"
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Revision as of 06:41, 2 October 2017
Introduction
500 bp of homologous arm＋His
Similarly, in order to knock out the gene called Sst2 which have the inhibition to the GpaI who play an important role in the signal transduction, The composite part is composed of the length of 500 bp of homologous arm and the marker of His, and it can replace the gene of Sst2 by homologous recombination, which can eliminate the influence of inhibition of sweetness signal transduction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1240
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1131
Illegal BglII site found at 1191 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1801
- 1000COMPATIBLE WITH RFC[1000]