Difference between revisions of "Part:BBa K2368011"

 
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__NOTOC__
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<h1>Introduction</h1>
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<partinfo>BBa_K2368011 short</partinfo>
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<p>In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct T1R3-flag tag. This tag can be recognized by specific monoclonal antibodies then we use fluorescence second antibody to interact with monoclonal antibodies to show the T1R3’s expression and location.</p>
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<p>Because the T1R3 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein αsubunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R3 and flag tag. And hope to show the location of T1R3 through immunofluorescence for detecting flag tag.</p>
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===Usage and Biology===
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<h2>Sequence and Features</h2>
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<partinfo>BBa_K2368011 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2368011 parameters</partinfo>
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Revision as of 06:18, 2 October 2017


Introduction

Flag+T1R3 overlap

In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct T1R3-flag tag. This tag can be recognized by specific monoclonal antibodies then we use fluorescence second antibody to interact with monoclonal antibodies to show the T1R3’s expression and location.

Because the T1R3 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein αsubunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R3 and flag tag. And hope to show the location of T1R3 through immunofluorescence for detecting flag tag.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]