Difference between revisions of "Part:BBa K2215005"

Latest revision as of 03:59, 2 October 2017

Putative Symbiodinium Terminator

This is a putative Symbiodinium terminator that is believed to work best in Symbiodinium due to its unique polyadenylation signal, AAAAG/C, instead of the typical eukaryotic polyadenylation signal, AAUAAA. This terminator should allow for "more expression and stability of transgenes introduced into Symbiodinium." (Levin, et al., 2017) Because this terminator was pulled from a Symbiodinium genome, it should therefore be utilized in Symbiodinium only or can be optimized for other species. This part will be characterized in a Chlamydomonas vector, pBR9 mCherry Cr.

Secondary Structure

File:Mfold-K2215005-1.png

Measurement

[http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

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 <partinfo>BBa_K2215005 short</partinfo>
-* This is a putative Symbiodinium terminator that is believed to work best in Symbiodinium due to its unique polyadenylation signal, AAAAG/C, instead of the typical eukaryotic polyadenylation signal, AAUAAA. This terminator should allow for "more expression and stability of transgenes introduced into Symbiodinium." (Levin, et al., 2017)
+* This is a putative Symbiodinium terminator that is believed to work best in Symbiodinium due to its unique polyadenylation signal, AAAAG/C, instead of the typical eukaryotic polyadenylation signal, AAUAAA. This terminator should allow for "more expression and stability of transgenes introduced into Symbiodinium." (Levin, et al., 2017) Because this terminator was pulled from a Symbiodinium genome, it should therefore be utilized in Symbiodinium only or can be optimized for other species. This part will be characterized in a Chlamydomonas vector, pBR9 mCherry Cr.
 |}