Difference between revisions of "Part:BBa K2379005"
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<partinfo>BBa_K2379005 short</partinfo> | <partinfo>BBa_K2379005 short</partinfo> | ||
− | The RNA thermometer (BBa_K115001) is | + | The RNA thermometer (BBa_K115001) is under the regulation of pLAC promoter (BBa_R0011). Following the RNA thermometer, the construct contains GFP(BBa_E0040) as the reporter which is followed by the double terminator (BBa_B0014). Expression of GFP is regulated by the RNA thermometer and hence will be produced only at 42degree celsius. |
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Revision as of 12:56, 1 October 2017
RNA thermometer (FourU) with GFP under the regulation of pLAC
The RNA thermometer (BBa_K115001) is under the regulation of pLAC promoter (BBa_R0011). Following the RNA thermometer, the construct contains GFP(BBa_E0040) as the reporter which is followed by the double terminator (BBa_B0014). Expression of GFP is regulated by the RNA thermometer and hence will be produced only at 42degree celsius.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 765