Difference between revisions of "Part:BBa K2379011"

 
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<partinfo>BBa_K2379011 short</partinfo>
 
<partinfo>BBa_K2379011 short</partinfo>
  
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Uses the adaptor(BBa_K2379008), which is under the influence of the pLac promoter(BBa_R0011) followed by the RNA pH meter(BBa_K2379002). Following the adaptor is the reporter protein GFP (BBa_E0040) followed by the double terminator(BBa_B0014)
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This construct consists of the adaptor(BBa_K2379008), which is under the influence of the pLac promoter(BBa_R0011). In addition to the adaptor, the RNA pHmeter with the natural scar site (BBa_K2379002) is used to regulate the transcription of the genes downstream. Following the adaptor is the reporter protein GFP (BBa_E0040) and the double terminator(BBa_B0014). Since it is regulated by the RNA pHmeter, the circuit will get activated and GFP will be produced only at pH 8.4
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:43, 1 October 2017


Adaptor with RNA pH meter

This construct consists of the adaptor(BBa_K2379008), which is under the influence of the pLac promoter(BBa_R0011). In addition to the adaptor, the RNA pHmeter with the natural scar site (BBa_K2379002) is used to regulate the transcription of the genes downstream. Following the adaptor is the reporter protein GFP (BBa_E0040) and the double terminator(BBa_B0014). Since it is regulated by the RNA pHmeter, the circuit will get activated and GFP will be produced only at pH 8.4

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 181
    Illegal BsaI.rc site found at 1219