Difference between revisions of "Part:BBa K2379009"
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<partinfo>BBa_K2379009 short</partinfo> | <partinfo>BBa_K2379009 short</partinfo> | ||
− | + | This part consists of the adaptor(BBa_K2379008), which is under the influence of the pLac promoter(BBa_R0011). In addition to the adaptor, a constitutive ribosome binding site(BBa_B0034) is used to regulate the transcription of the genes downstream. Following the adaptor is the reporter protein GFP (BBa_E0040) and the double terminator(BBa_B0014). Since it is regulated by a constitutive RBS, the construct will be active at all times. | |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 12:34, 1 October 2017
Adaptor with constitutive RBS
This part consists of the adaptor(BBa_K2379008), which is under the influence of the pLac promoter(BBa_R0011). In addition to the adaptor, a constitutive ribosome binding site(BBa_B0034) is used to regulate the transcription of the genes downstream. Following the adaptor is the reporter protein GFP (BBa_E0040) and the double terminator(BBa_B0014). Since it is regulated by a constitutive RBS, the construct will be active at all times. Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1028