Difference between revisions of "Part:BBa K2379002"
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<partinfo>BBa_K2379002 short</partinfo> | <partinfo>BBa_K2379002 short</partinfo> | ||
− | The RNA pHmeter is used for pH-sensitive translational regulation and initiates translation at pH 8. | + | The RNA pHmeter is used for pH-sensitive translational regulation and initiates translation at pH 8.4. It is based on the RNA encoding gene, SraF from E.coli that is a part of the 5' UTR of the alx locus and brings about alx expression only under extremely alkaline conditions. |
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Revision as of 12:11, 1 October 2017
RNA pHmeter with natural scar site
The RNA pHmeter is used for pH-sensitive translational regulation and initiates translation at pH 8.4. It is based on the RNA encoding gene, SraF from E.coli that is a part of the 5' UTR of the alx locus and brings about alx expression only under extremely alkaline conditions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 118