Difference between revisions of "Part:BBa K2309005:Design"

 
(Design Notes)
 
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===Design Notes===
 
===Design Notes===
This part is for detection of expression efficiency of agr-regulated system.
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This part is for detection of expression efficiency of agr-regulated system, hence, we combined BBa_K515005 and BBa_2309003 together. Moreover, the sensing system for AIP has been optimized for Lactococcus lactis.
 
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===Source===
 
===Source===
  
Superfolder GFP is derived from BBa_K515005 and AIP-induced agr-regulated system is from another part of our team, which is numbered BBa_K2309004.
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Superfolder GFP is derived from BBa_K515005 and AIP-induced agr-regulated system is from another part of our team, which is numbered BBa_K2309003.
  
 
===References===
 
===References===

Latest revision as of 12:20, 29 September 2017


Manifestation of AIP induced expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1267
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2325
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is for detection of expression efficiency of agr-regulated system, hence, we combined BBa_K515005 and BBa_2309003 together. Moreover, the sensing system for AIP has been optimized for Lactococcus lactis.

Source

Superfolder GFP is derived from BBa_K515005 and AIP-induced agr-regulated system is from another part of our team, which is numbered BBa_K2309003.

References