Difference between revisions of "Part:BBa K2255007"
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The signal sequence is crucial for the excretion of p3 in the periplasm. We choose to use the one coming from M13 as we use ''E. coli'' to produce our phage. In order to be functional, the signal peptide must be cut down from the rest of the protein. Thus, we must add the cleavage site.  
 
The signal sequence is crucial for the excretion of p3 in the periplasm. We choose to use the one coming from M13 as we use ''E. coli'' to produce our phage. In order to be functional, the signal peptide must be cut down from the rest of the protein. Thus, we must add the cleavage site.  
  
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This part was design with Freiburg (RFC[25]) extension. Thus, it contain the restriction site  NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.
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This part was design with Freiburg ([https://parts.igem.org/Assembly_standard_25 Rfc25]) extension. Thus, it contain the restriction site  NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.
  
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===Usage and Biology===
 
===Usage and Biology===
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This biobrick was create to be associated with attachment protein biobrick from our collection. To see all the part from this collection, you can check out the first part of this collection : [https://parts.igem.org/Part:BBa_K2255008 BBa_K2255008].
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Revision as of 16:26, 26 September 2017


Signal sequence of p3 from M13 (Rfc25)

The signal sequence is crucial for the excretion of p3 in the periplasm. We choose to use the one coming from M13 as we use E. coli to produce our phage. In order to be functional, the signal peptide must be cut down from the rest of the protein. Thus, we must add the cleavage site.

This part was design with Freiburg (Rfc25) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.

Usage and Biology

This biobrick was create to be associated with attachment protein biobrick from our collection. To see all the part from this collection, you can check out the first part of this collection : BBa_K2255008.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]