Difference between revisions of "Part:BBa K2273108"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K2273108 short</partinfo> | <partinfo>BBa_K2273108 short</partinfo> | ||
| − | + | The <i>blaR1</i> gene is part of the Beta-Lactam Biosensor project of [http://2017.igem.org/Team:TU_Dresden iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].<br><br> | |
| − | + | This gene is part of the <i>bla</i> operon found in <i>Staphylococcus aureus</i> and encodes a receptor that localizes in the inner cell membrane and can bind beta-lactam antibiotics, Uniprot [http://www.uniprot.org/uniprot/P18357]).<br><br> | |
| + | ===Beta Lactam Biosensor | ||
| + | Worldwide, multidrug-resistant germs are on the rise and provoke the intensive search for novel effective compounds. To simplify the search for new antibiotics and to track the antibiotic pollution in water samples, whole cell biosensors constitute a helpful investigative tool. In this subproject, we developed a functional and complete heterologous beta-lactam biosensor in Bacillus subtilis. By the time these specified cells sense a compound of the beta-lactam family, they will respond by producing a measurable luminescence signal. We further investigated the detection spectrum of the biosensor by testing different beta-lactam antibiotics from various subclasses. For increased control and easy handling of the biosensor strain during a potential field application, we demonstrate that the encapsulation of the cells into Peptidosomes is quite advantageous. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | This Part serves as a sensing unit of the beta-lactam biosensor. The BlaR1 receptor is able to bind various beta-lactam antibiotics. After binding of a respective ligand, a signal is transducer into the bacterial cytoplasm. This Protein therefore harbours transmembrane helices and a Penicillin Binding domain (amino acid 331-585). | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 13:26, 25 September 2017
BlaR1 Beta-Lactam Receptor derived from Staphylococcus aureus N315
The blaR1 gene is part of the Beta-Lactam Biosensor project of [http://2017.igem.org/Team:TU_Dresden iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].
This gene is part of the bla operon found in Staphylococcus aureus and encodes a receptor that localizes in the inner cell membrane and can bind beta-lactam antibiotics, Uniprot [http://www.uniprot.org/uniprot/P18357]).
===Beta Lactam Biosensor
Worldwide, multidrug-resistant germs are on the rise and provoke the intensive search for novel effective compounds. To simplify the search for new antibiotics and to track the antibiotic pollution in water samples, whole cell biosensors constitute a helpful investigative tool. In this subproject, we developed a functional and complete heterologous beta-lactam biosensor in Bacillus subtilis. By the time these specified cells sense a compound of the beta-lactam family, they will respond by producing a measurable luminescence signal. We further investigated the detection spectrum of the biosensor by testing different beta-lactam antibiotics from various subclasses. For increased control and easy handling of the biosensor strain during a potential field application, we demonstrate that the encapsulation of the cells into Peptidosomes is quite advantageous.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]