Difference between revisions of "Part:BBa K2273066:Design"

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===References===
 
===References===
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Ulf Brockmeier, Michael Caspers, Roland Freudl, Alexander Jockwer, Thomas Noll and Thorsten Eggert “Systematic Screening of All Signal Peptides from Bacillus subtilis: A Powerful Strategy in Optimizing Heterologous Protein Secretion in Gram-positive Bacteria“ Journal of Molecular Biology 362 (2006): 393-402. [https://www.ncbi.nlm.nih.gov/pubmed/16930615 PubMed]

Revision as of 19:10, 22 September 2017


SpoIID signal peptide of B. subtilis lytic transglycosylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design

tba

Source

The SpoIID signal peptide of B. subtilis lytic transglycosylase was amplified via PCR from the B. subtilis wild type W168 genome using the primers listed below.

SpoIID_SP fwd gatcGAATTCGCGGCCGCTTCTAGATAAGGAGGTCAAAAATGAAACAATTCGCAATCACACTAT
SpoIID_SP rev gatcTCTGCAGCGGCCGCTACTAGTATTAACCGGTGGCCCCCGCTTCCTTATT

Following amplification, the signal peptide was digested using EcoRI and PstI and ligated into pSB1C3.

References

Ulf Brockmeier, Michael Caspers, Roland Freudl, Alexander Jockwer, Thomas Noll and Thorsten Eggert “Systematic Screening of All Signal Peptides from Bacillus subtilis: A Powerful Strategy in Optimizing Heterologous Protein Secretion in Gram-positive Bacteria“ Journal of Molecular Biology 362 (2006): 393-402. PubMed