Difference between revisions of "Part:BBa K2404000"

Revision as of 11:39, 22 September 2017

A Thyroid Stimulating Hormone antagonist without His-Tags

This is a coding sequence of a gene that produces a protein that competitively inhibits thyroid stimulating hormone (TSH). It also inhibits auto-immune antibodies that result in Graves' disease. This protein is modified to contain a linker region between two sub units. These sub units are the alpha sub unit which is shared between many human hormones including some sex hormones such as human chorionic gonadotropin (hCG), and the beta sub unit which is specific to TSH. These sub units have been joined in this modified protein by a CTD linker to produce one protein that mimics natural TSH in the body. However, the TSH antagonist lacks glycosylation groups that usually exist on both sub units. This antagonist binds to the TSH receptor but does not activate it as a result. This part would be useful when used as the coding sequence of a gene construct to create a drug that treats hyperthyroidism. This part is contained within the pSB1C3 plasmid.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 540
Illegal PstI site found at 582
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 540
Illegal PstI site found at 582
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 607
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 540
Illegal PstI site found at 582
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 540
Illegal PstI site found at 582
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 794

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2404000 short</partinfo>
-This is a coding sequence of a gene that produces a protein that competitively inhibits thyroid stimulating hormone (TSH). It also inhibits auto-immune antibodies that result in Graves' disease. This protein is modified to contain a linker region between two sub units. These sub units are the alpha sub unit which is shared between many human hormones including some sex hormones such as human chorionic gonadotropin (hCG), and the beta sub unit which is specific to TSH. These sub units have been joined in this modified protein by a CTD linker to produce one protein that mimics natural TSH in the body. However, the TSH antagonist lacks glycosylation groups that usually exist on both sub units. This antagonist binds to the TSH receptor but does not activate it as a result. This part would be useful when used as the coding sequence of a gene construct to create a drug that treats hyperthyroidism.
+This is a coding sequence of a gene that produces a protein that competitively inhibits thyroid stimulating hormone (TSH). It also inhibits auto-immune antibodies that result in Graves' disease. This protein is modified to contain a linker region between two sub units. These sub units are the alpha sub unit which is shared between many human hormones including some sex hormones such as human chorionic gonadotropin (hCG), and the beta sub unit which is specific to TSH. These sub units have been joined in this modified protein by a CTD linker to produce one protein that mimics natural TSH in the body. However, the TSH antagonist lacks glycosylation groups that usually exist on both sub units. This antagonist binds to the TSH receptor but does not activate it as a result. This part would be useful when used as the coding sequence of a gene construct to create a drug that treats hyperthyroidism. This part is contained within the pSB1C3 plasmid.
 <!-- Add more about the biology of this part here