Difference between revisions of "Part:BBa K2332000:Experience"
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===Applications of BBa_K2332000=== | ===Applications of BBa_K2332000=== | ||
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| + | We designed this part to test our bacterial cell adhesion hypothesis. We predicted that SpyTag and SpyCatcher will form a covalent bond upon contact, which is what we desire in our gene circuit. | ||
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| + | To test this visually, we express SpyCatcher on the surface of E. Coli with Intimin, a surface display protein. When the GFP-SpyTag fusion protein is added, we can observe the area around the cells to present green fluorescence as opposed to the control cells that were only exposed to the GFP protein without the SpyTag. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 18:09, 3 September 2017
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2332000
We designed this part to test our bacterial cell adhesion hypothesis. We predicted that SpyTag and SpyCatcher will form a covalent bond upon contact, which is what we desire in our gene circuit.
To test this visually, we express SpyCatcher on the surface of E. Coli with Intimin, a surface display protein. When the GFP-SpyTag fusion protein is added, we can observe the area around the cells to present green fluorescence as opposed to the control cells that were only exposed to the GFP protein without the SpyTag.
User Reviews
UNIQ651606bf66ad09cb-partinfo-00000000-QINU UNIQ651606bf66ad09cb-partinfo-00000001-QINU