Difference between revisions of "Part:BBa K2255009"
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This part is sequence coding for the D1 and D2 of the protein 3 of NgoΦ6, a filamentous phage that target N.gonorrhoeae. Those domains are required for M13 adsorption and entry. | This part is sequence coding for the D1 and D2 of the protein 3 of NgoΦ6, a filamentous phage that target N.gonorrhoeae. Those domains are required for M13 adsorption and entry. | ||
− | This part was design with Freiburg (RFC[25]) | + | This part was design with Freiburg (RFC[25]) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain. |
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Revision as of 13:07, 30 August 2017
p3_N.gonorrhoeae (Rfc25)
This part is sequence coding for the D1 and D2 of the protein 3 of NgoΦ6, a filamentous phage that target N.gonorrhoeae. Those domains are required for M13 adsorption and entry.
This part was design with Freiburg (RFC[25]) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]