Difference between revisions of "Part:BBa K2255011"
Line 4: | Line 4: | ||
This part is sequence coding for the D1 and D2 of the protein 3 of RSM1, a filamentous phage that target R.solanacearum. Those domains are required for phage adsorption and entry. | This part is sequence coding for the D1 and D2 of the protein 3 of RSM1, a filamentous phage that target R.solanacearum. Those domains are required for phage adsorption and entry. | ||
+ | |||
+ | This part was design with Freiburg (RFC[25]) as extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 13:00, 30 August 2017
p3_R.solanacearum_RSM1 (Rfc25)
This part is sequence coding for the D1 and D2 of the protein 3 of RSM1, a filamentous phage that target R.solanacearum. Those domains are required for phage adsorption and entry.
This part was design with Freiburg (RFC[25]) as extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]