Difference between revisions of "Part:BBa K1033916:Experience"
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| − | [[File:Amaj.PNG |center|thumb| | + | [[File:Amaj.PNG |center|thumb|350px|''<b>Fig.2</b> Vary pH attributed to different fluorescent intensity of RFP]] |
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<caption><p align="justify"><b>Table 1</b> Plate reader setting of fluorescent measurement</p></caption> | <caption><p align="justify"><b>Table 1</b> Plate reader setting of fluorescent measurement</p></caption> | ||
Revision as of 16:53, 26 August 2017
This experience page is provided so that any user may enter their experience using this part.
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Applications of BBa_K1033916
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UNIQ36833e4db31ee02e-partinfo-00000000-QINU UNIQ36833e4db31ee02e-partinfo-00000001-QINU
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Hong Kong-CUHK iGEM 2017 |
Fluorescent properties of amajLime Although amajLime is described as chromoprotein in main page, we characterised its spectral properties and found the max excitation and emission wavelength at 445 nm nd 485 nm respectively.
We transformed part BBa_K1033916 with constituitive promoter: J23100 in C41 and grew in 2XYT for 24 hours. Purifying the amajLime by Ion Exchange Chromatography and Hydrophobic Interaction Chromatography, we measured the fluoresece of purified amajLime, which is diluted to 10µg/100µl (total 200µl) in triplicates, in different buffers (ranges from pH2 to pH12). The result shows that the stability drops dramatically in condition below 6 and attains maxima fluorescence at pH 8.
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