Difference between revisions of "Part:BBa K1033916:Experience"
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<b> Fluorescent properties of amajLime </b> | <b> Fluorescent properties of amajLime </b> | ||
<p> Instead of chromorptein, we proposed that amajLime is a fluorescent protein with the property of showing pigmentation, like BBa_E1010. We charaterised spectral properties of amajLime and found its max excitation and emission wavelength at 445 nm nd 485 nm respectively.</p> | <p> Instead of chromorptein, we proposed that amajLime is a fluorescent protein with the property of showing pigmentation, like BBa_E1010. We charaterised spectral properties of amajLime and found its max excitation and emission wavelength at 445 nm nd 485 nm respectively.</p> | ||
| − | Fig.1 Emission and Excitation spectra | + | [[File:Ex-Em. amajlime.png|width='60%' valign='top'| |center|thumb|550px|''<b>Fig.1</b> Emission and Excitation spectra]] |
<br> | <br> | ||
<b> Charaterization of pH stability of amajLime </b> | <b> Charaterization of pH stability of amajLime </b> | ||
| − | <p>We grew C41 bacteria with parts BBa_K1033916 with constituitive promoter: J23100, in 2XYT for 24 hours. Purifying the amajLime by Ion Exchange Chromatography and Hydrophobic Interaction Chromatography, we measured the fluoresece of purified amajLime, which is diluted to 10µg/100µl (total 200µl) in triplicates, in different buffers (ranges from pH2 to pH12). The result shows that the stability drops dramatically in condition below 6 and attains maxima fluorescence at pH | + | <p>We grew C41 bacteria with parts BBa_K1033916 with constituitive promoter: J23100, in 2XYT for 24 hours. Purifying the amajLime by Ion Exchange Chromatography and Hydrophobic Interaction Chromatography, we measured the fluoresece of purified amajLime, which is diluted to 10µg/100µl (total 200µl) in triplicates, in different buffers (ranges from pH2 to pH12). The result shows that the stability drops dramatically in condition below 6 and attains maxima fluorescence at pH 8.</p> |
| − | Fig.2 Vary pH attributed to different fluorescent intensity of RFP | + | |
| + | <div align="center"> | ||
| + | [[File:Amaj.PNG |center|thumb|350px|''<b>Fig.2</b> Vary pH attributed to different fluorescent intensity of RFP]] | ||
<table cellpadding="2" border="1px" cellspacing="0" align="center" width="70%"> | <table cellpadding="2" border="1px" cellspacing="0" align="center" width="70%"> | ||
<caption><p align="justify"><b>Table 1</b> Plate reader setting of fluorescent measurement</p></caption> | <caption><p align="justify"><b>Table 1</b> Plate reader setting of fluorescent measurement</p></caption> | ||
Revision as of 16:41, 26 August 2017
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Hong Kong-CUHK iGEM 2017 |
Fluorescent properties of amajLime Instead of chromorptein, we proposed that amajLime is a fluorescent protein with the property of showing pigmentation, like BBa_E1010. We charaterised spectral properties of amajLime and found its max excitation and emission wavelength at 445 nm nd 485 nm respectively.
We grew C41 bacteria with parts BBa_K1033916 with constituitive promoter: J23100, in 2XYT for 24 hours. Purifying the amajLime by Ion Exchange Chromatography and Hydrophobic Interaction Chromatography, we measured the fluoresece of purified amajLime, which is diluted to 10µg/100µl (total 200µl) in triplicates, in different buffers (ranges from pH2 to pH12). The result shows that the stability drops dramatically in condition below 6 and attains maxima fluorescence at pH 8.
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