Difference between revisions of "Help:Materials/Antibiotic Stocks"

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{{HelpPage/MainLinks}}
 
{{HelpPage/MainLinks}}
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[[Category:Materials]]
 
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At iGEM HQ we prepare 1000x stock solutions of antibiotics which are stored at -20C until use.
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At iGEM HQ we prepare '''1000x stock solutions''' of antibiotics which are stored at -20C until use. Antibiotics are also color coded.
  
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*Ampicillin
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====Ampicillin====
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**100mg/ml in 50% ethanol
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*'''Stock:''' 100mg/ml in 50% ethanol
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*Chloramphenicol
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*'''Working:''' 100ug/ml
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**35mg/ml in 100% ethanol
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*'''Color Code:''' Orange
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*Kanamycin
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====Chloramphenicol====
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**35mg/ml in distilled water
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*'''Stock:''' 35mg/ml in 100% ethanol
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*Tetracycline
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*'''Working:''' 35ug/ml
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**15mg/ml in 50% ethanol
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*'''Color Code:''' Green
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**Light-sensitive! Store in light-sensitive bottles or wrap with aluminum foil.
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====Kanamycin====
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*'''Stock:''' 35mg/ml in distilled water
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*'''Working:''' 35ug/ml
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*'''Color Code:''' Red
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*'''Notes:''' Stocks are stored in 1ml aliquots, and thawed before use
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====Tetracycline====
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*'''Stock:''' 15mg/ml in 50% ethanol
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*'''Working:''' 15ug/ml
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*'''Color Code:''' Yellow
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*'''Notes:''' Light-sensitive! Store in light-sensitive bottles or wrap with aluminum foil.

Latest revision as of 19:41, 21 July 2017

At iGEM HQ we prepare 1000x stock solutions of antibiotics which are stored at -20C until use. Antibiotics are also color coded.

Ampicillin

  • Stock: 100mg/ml in 50% ethanol
  • Working: 100ug/ml
  • Color Code: Orange

Chloramphenicol

  • Stock: 35mg/ml in 100% ethanol
  • Working: 35ug/ml
  • Color Code: Green

Kanamycin

  • Stock: 35mg/ml in distilled water
  • Working: 35ug/ml
  • Color Code: Red
  • Notes: Stocks are stored in 1ml aliquots, and thawed before use

Tetracycline

  • Stock: 15mg/ml in 50% ethanol
  • Working: 15ug/ml
  • Color Code: Yellow
  • Notes: Light-sensitive! Store in light-sensitive bottles or wrap with aluminum foil.