Difference between revisions of "Help:BioBrick Assembly"

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[[Category:BioBrick RFC 10]]
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Physical parts in the DNA Repository have been designed to be assembled into systems using normal cloning techniques based on restriction enzymes, purification, ligation, and transformation - with a twist: BioBrick parts are [[Assembly:Composable|composable]].  The result of assembling two parts is a new part that may be used in future assemblies.
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Physical parts in the DNA Repository have been designed to be assembled into systems using normal cloning techniques based on restriction enzymes, purification, ligation, and transformation - with a twist: BioBrick parts are [[Assembly:Composable|composable]].  The result of assembling two parts is a new part that may be used in future assemblies. Certain [[Assembly:RBS-CDS issues|RBS-CDS issues]] must also be considered.
  
 
==Standard Assembly==
 
==Standard Assembly==
 
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|[https://parts.igem.org/wiki/index.php?title=Help:BioBrick_Assembly  https://static.igem.org/mediawiki/parts/f/ff/Bricks.png]
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[https://parts.igem.org/wiki/index.php?title=Assembly:Standard_assembly https://static.igem.org/mediawiki/parts/f/ff/Bricks.png]
 
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Standard Assembly chat - The classical method of doing BioBrick assemblies, done at the lab bench
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BioBrick parts can be assembled to form useful devices, through a process often referred to as 'Standard Assembly'. This uses normal cloning techniques based on restriction enzymes, purification, ligation, and transformation. Find out more about [[Assembly:Standard assembly|Standard Assembly]].
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*[[Assembly:Standard assembly|Standard Assembly]]
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*[[Assembly|Assembly of Registry Parts]]
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*[[Characterization of Parts]] - Part ontology and measurements
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*[[Assembly:RBS-CDS issues|RBS-CDS Design Issues]]
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==Parallel Assembly==
 
==Parallel Assembly==
 
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|[[Image:Rolling assembly.jpg|80px]]
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{{Click || image=Rolling assembly.jpg| | link=Assembly:Rolling assembly |width=80px | height=80px }}
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BioBrick systems may contain many parts. One could spend many weeks building a 50-part system by assembling the first two parts, adding the third part, adding the fourth part, and so on. However, because BioBrick assembly is composable, assembly need not be done sequentially - [[Assembly:Rolling assembly|Find out more...]]
 
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==Automated Assembly==
 
==Automated Assembly==
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{{Click || image=Robotic.png| | link=Assembly:Robotic assembly |width=100px | height=100px }}
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Automated assemblies, currently in late stage development at iGEM labs
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*[[Assembly:Robotic assembly|Robotic Assembly]]
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Automated assemblies, currently in late stage development at iGEM labs. find out more [[Assembly:Robotic assembly|here]].
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*[[Assembly:Parts in plasmids|Parts in Plasmids]]
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*[[Assembly:Restriction enzymes|Restriction Enzymes]]
 
 
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Latest revision as of 19:48, 16 June 2017


Physical parts in the DNA Repository have been designed to be assembled into systems using normal cloning techniques based on restriction enzymes, purification, ligation, and transformation - with a twist: BioBrick parts are composable. The result of assembling two parts is a new part that may be used in future assemblies. Certain RBS-CDS issues must also be considered.

Standard Assembly

Bricks.png

BioBrick parts can be assembled to form useful devices, through a process often referred to as 'Standard Assembly'. This uses normal cloning techniques based on restriction enzymes, purification, ligation, and transformation. Find out more about Standard Assembly.

Parallel Assembly

BioBrick systems may contain many parts. One could spend many weeks building a 50-part system by assembling the first two parts, adding the third part, adding the fourth part, and so on. However, because BioBrick assembly is composable, assembly need not be done sequentially - Find out more...

Automated Assembly

Automated assemblies, currently in late stage development at iGEM labs. find out more here.