Difference between revisions of "Part:BBa K2333005:Design"
CiCi Zheng (Talk | contribs) (→References) |
|||
| (2 intermediate revisions by the same user not shown) | |||
| Line 15: | Line 15: | ||
This tag was derived using mutagenesis from the endogenous Lon degraded tags from the bacteria Mycoplasma florum by Collins et al. 2014 "Tunable Protein Degradation in Bacteria". This specific sequence was synthesized by IDT. | This tag was derived using mutagenesis from the endogenous Lon degraded tags from the bacteria Mycoplasma florum by Collins et al. 2014 "Tunable Protein Degradation in Bacteria". This specific sequence was synthesized by IDT. | ||
| + | |||
| + | We use the website www.jcat.de to optimize the codon sequence for E. coli. | ||
===References=== | ===References=== | ||
| + | [1] Cameron DE, Collins JJ. Tunable protein degradation in bacteria. Nature Biotechnology. 2014;32(12):1276–1281. | ||
| + | |||
| + | [2] Gur E, Sauer RT. Evolution of the ssrA degradation tag in Mycoplasma: Specificity switch to a different protease. Proceedings of the National Academy of Sciences. 2008;105(42):16113–16118. | ||
Latest revision as of 16:33, 16 June 2017
mf-Lon Protein Degradation Tag E (medium-weak)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This specific sequence was created by E. coli codon optimizing the amino acid sequence provided in the supplement by Collins et al, as a DNA sequence was not provided.
Source
This tag was derived using mutagenesis from the endogenous Lon degraded tags from the bacteria Mycoplasma florum by Collins et al. 2014 "Tunable Protein Degradation in Bacteria". This specific sequence was synthesized by IDT.
We use the website www.jcat.de to optimize the codon sequence for E. coli.
References
[1] Cameron DE, Collins JJ. Tunable protein degradation in bacteria. Nature Biotechnology. 2014;32(12):1276–1281.
[2] Gur E, Sauer RT. Evolution of the ssrA degradation tag in Mycoplasma: Specificity switch to a different protease. Proceedings of the National Academy of Sciences. 2008;105(42):16113–16118.