Difference between revisions of "Part:BBa M50063:Design"

Latest revision as of 06:19, 12 June 2017

Ara h2 Production Device

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 212
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 212
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 376
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 212
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 212
1000
COMPATIBLE WITH RFC[1000]

Design Notes

This DNA devices has six parts: An IPTG inducible inducible T5 promoter sourced from the iGEM registry of parts; a strong ribosomal site sourced from the iGEM registry of parts; the Ara h2 gene taken from the Ara h2 protein sequence in the paper Production of peanut antigen in L.lactis, Glenting, et al., reverse translated from protein sequence to a genetic sequence, and codon optimized for the E. coli organism; a 6x histidine tag attached to the end of the Ara h2 sequence for identification with a western blot sourced from DNA 2.0; and a T5 terminator sourced from DNA 2.0; a giii secretion tag sourced from DNA 2.0 and added directly before the Ara h2 sequence to hopefully aid in Ara h2 secretion. This device was synthesized into a plasmid containing a kanamycin resistance cassette by DNA 2.0.

Source

Plasmid name: pMW_IJ_export

DNA2.0 Gene #: PD441-gIII

Organism: E. coli

Device type: Actuator

Glycerol stock barcode: 0133027184

Box label: BioE44 S17

References

Glenting, J. et al. Production of Recombinant Peanut Allergen Ara h 2 using Lactococcus lactis. Microb. Cell Factories 6, 28 (2007).

@@ Line 7: / Line 7: @@
 ===Design Notes===
-This DNA devices had six parts: An IPTG inducible inducible T5 promoter sourced from the iGEM registry of parts; a strong ribosomal site sourced from the iGEM registry of parts; the Ara h2 gene taken from the Ara h2 protein sequence in the paper Production of peanut antigen in L.lactis, Glenting, et al., reverse translated from protein sequence to a genetic sequence, and codon optimized for the E. coli organism; a 6x histidine tag attached to the end of the Ara h2 sequence for identification with a western blot sourced from DNA 2.0; and a T5 terminator sourced from DNA 2.0; a giii secretion tag sourced from DNA 2.0 and added directly before the Ara h2 sequence to hopefully aid in Ara h2 secretion. The two devices were synthesized into plasmids containing a kanamycin resistance cassette by DNA 2.0.
+This DNA devices has six parts: An IPTG inducible inducible T5 promoter sourced from the iGEM registry of parts; a strong ribosomal site sourced from the iGEM registry of parts; the Ara h2 gene taken from the Ara h2 protein sequence in the paper Production of peanut antigen in L.lactis, Glenting, et al., reverse translated from protein sequence to a genetic sequence, and codon optimized for the E. coli organism; a 6x histidine tag attached to the end of the Ara h2 sequence for identification with a western blot sourced from DNA 2.0; and a T5 terminator sourced from DNA 2.0; a giii secretion tag sourced from DNA 2.0 and added directly before the Ara h2 sequence to hopefully aid in Ara h2 secretion. This device was synthesized into a plasmid containing a kanamycin resistance cassette by DNA 2.0.
 ===Source===
-Glenting, J. et al. Production of Recombinant Peanut Allergen Ara h 2 using Lactococcus lactis. Microb. Cell Factories 6, 28 (2007).
+Plasmid name: pMW_IJ_export
+DNA2.0 Gene #: PD441-gIII
+Organism: E. coli
+Device type: Actuator
+Glycerol stock barcode: 0133027184
+Box label: BioE44 S17
 ===References===
 Glenting, J. et al. Production of Recombinant Peanut Allergen Ara h 2 using Lactococcus lactis. Microb. Cell Factories 6, 28 (2007).