Difference between revisions of "Part:BBa J176013"

Revision as of 23:33, 2 March 2017

VP64

Tetrameric VP16 transcription activator domain

Usage and Biology

The VP64 Activation Domain Module is often fused to DNA binding domains to make strong synthetic transcription factors. Figure adapted from Beerli et al., 1998.

• Mammalian expression vector required
• Protein domain; requires promoter, start codon, stop codon, and polyA signal for proper expression
• VP64 doesn't do much as a free-floating domain. Fuse it to a DNA binding domain for the best results
• Uniprot entry UL48 (VP16) - VP64 is a 4x tandem array of amino acids 438-448
  

VP64 is a transcriptional activator composed of four tandem copies of VP16 (Herpes Simplex Viral Protein 16, amino acids 437-447*: DALDDFDLDML) connected with glycine-serine (GS) linkers. When fused to another protein domain that can bind near the promoter of a gene, VP64 acts as a strong transcriptional activator. This module is a classic molecular biology tool. See the References section for some history on VP16 and VP64. *Note: numbering excludes the starting methionine so the first a.a. is actually 438, consistent with the Uniprot entry UL48.

Sequence and Features