Difference between revisions of "Part:BBa J100313"

Latest revision as of 15:43, 19 January 2017

Scrambled OmpR Partial Promoter (first 70 bp)

We scrambled the first 70 base pairs of the OmpR promoter (R0082). This should disrupt the transcription factor binding sites such that the promoter is no longer functional. We will insert this modified promoter in actClone (J100204) to serve as a negative control when testing the effects of modifications to the promoter. We have indicated the location of binding sites in the wt promoter as annotated in R0082, the scrambling means they are unlikely to still bind.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 32
Illegal SpeI site found at 59
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 32
Illegal SpeI site found at 59
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 32
Illegal SpeI site found at 59
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 32
Illegal SpeI site found at 59
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_J100313 short</partinfo>
-We scrambled the first 70 base pairs of the OmpR promoter (R0082). This should disrupt the transcription factor binding sites such that the promoter is no longer functional. We will insert this modified promoter in actClone (J100204) to serve as a negative control when testing the effects of modifications to the promoter.
+We scrambled the first 70 base pairs of the OmpR promoter (R0082). This should disrupt the transcription factor binding sites such that the promoter is no longer functional. We will insert this modified promoter in actClone (J100204) to serve as a negative control when testing the effects of modifications to the promoter. We have indicated the location of binding sites in the wt promoter as annotated in R0082, the scrambling means they are unlikely to still bind.
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