Difference between revisions of "Part:BBa M50051:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | ===Plasmid Map=== | ||
| + | [[File:BBa_M50051_Plasmid_Map.png|thumb|500px]] | ||
| + | We did not run into any major design considerations after analyzing our construct using Gene Designer. Our inspiration for this project stemmed from the literature suggesting that AI-2 is a quorum sensing pathway, that could possibly allow control of bacterial growth(see reference 1). | ||
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| + | 1. Xavier KB and Bassler BL. 2005. Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli. J Bacteriol. 187(1): 238-248. | ||
Latest revision as of 07:59, 12 December 2016
IPTG Inducible Plasmid for the Expression of LsrR Repressor
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1134
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1134
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1134
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1134
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Plasmid Map
We did not run into any major design considerations after analyzing our construct using Gene Designer. Our inspiration for this project stemmed from the literature suggesting that AI-2 is a quorum sensing pathway, that could possibly allow control of bacterial growth(see reference 1).
Source
The genetic sequence of the LsrR gene was sourced through the UniProt database. Other parts were sourced through DNA 2.0 under the plasmid name pD444-CH.
References
1. Xavier KB and Bassler BL. 2005. Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli. J Bacteriol. 187(1): 238-248.