Difference between revisions of "Part:BBa M50050"

Latest revision as of 07:34, 12 December 2016

IPTG-Inducible LuxS Expression in E. coli for Controlling Growth Rates

When transfected into E. coli, this plasmid will augment it's ability to express the LuxS synthase protein, allowing for differing production of Autoinducer-2. The expression of this plasmid is controlled by inducing with IPTG, allowing for artificial control of increased AI-2 levels. The promoter, the RBS, the his-tag, and the terminator were sourced through DNA 2.0. However, we have included parts from the Parts Registry in this project. The promoter is a T5 IPTG-inducible promoter that allows for the control of the expression of LuxS by varying the induction. The his-tag will allow us to test the expression through a Western blot.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 696
Illegal PstI site found at 202
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 696
Illegal PstI site found at 202
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 307
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 696
Illegal PstI site found at 202
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 696
Illegal PstI site found at 202
Illegal AgeI site found at 405
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_M50050 short</partinfo>
-When transfected into E. coli, this plasmid will augment it's ability to express the LuxS synthase protein, allowing for differing production of Autoinducer-2. The expression of this plasmid is controlled by inducing with IPTG, allowing for artificial control of increased AI-2 levels.
+When transfected into E. coli, this plasmid will augment it's ability to express the LuxS synthase protein, allowing for differing production of Autoinducer-2. The expression of this plasmid is controlled by inducing with IPTG, allowing for artificial control of increased AI-2 levels. The promoter, the RBS, the his-tag, and the terminator were sourced through DNA 2.0. However, we have included parts from the Parts Registry in this project. The promoter is a T5 IPTG-inducible promoter that allows for the control of the expression of LuxS by varying the induction. The his-tag will allow us to test the expression through a Western blot.
 <!-- Add more about the biology of this part here