Difference between revisions of "Part:BBa M50041:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | The second half of our genetic construct was “EColi_Promoter_Cassette,” containing strong RBS, the sequence for green fluorescent protein(GFP) with a terminator immediately following it, an ampicillin resistance marker, and a high copy number origin of replication (ORI). “EColi_Promoter_Cassette” was obtained from Dr. Kara Rogers. | |
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===References=== | ===References=== | ||
+ | “cusR response regulator in two-component regulatory system with cusS [Escherichia coli str. K-12 substr. MG1655”. (2016, Sep. 10). NCBI Reference Sequences.<BR><BR>Zahid N, Zulfiqar S, Shakoori AR. (2012, March). Functional analysis of cus operon promoter of Klebsiella pneumoniae using E. coli lacZ assay. Gene, 495(1):81-88. <BR><BR>Loftin IR, et al. (2005, July). A novel copper-binding fold for the periplasmic copper resistance protein cusF. Biochemistry, 44(31):10533-10540. |
Revision as of 23:52, 11 December 2016
Positive feedback cusR producer (mutated)
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 53
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 53
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 53
Illegal AgeI site found at 243 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The second half of our genetic construct was “EColi_Promoter_Cassette,” containing strong RBS, the sequence for green fluorescent protein(GFP) with a terminator immediately following it, an ampicillin resistance marker, and a high copy number origin of replication (ORI). “EColi_Promoter_Cassette” was obtained from Dr. Kara Rogers.
Source
source
References
“cusR response regulator in two-component regulatory system with cusS [Escherichia coli str. K-12 substr. MG1655”. (2016, Sep. 10). NCBI Reference Sequences.
Zahid N, Zulfiqar S, Shakoori AR. (2012, March). Functional analysis of cus operon promoter of Klebsiella pneumoniae using E. coli lacZ assay. Gene, 495(1):81-88.
Loftin IR, et al. (2005, July). A novel copper-binding fold for the periplasmic copper resistance protein cusF. Biochemistry, 44(31):10533-10540.