Difference between revisions of "Part:BBa M50039:Design"
Abbiemcnulty (Talk | contribs) (→Source) |
Abbiemcnulty (Talk | contribs) (→Source) |
||
Line 11: | Line 11: | ||
===Source=== | ===Source=== | ||
− | |||
===References=== | ===References=== |
Revision as of 23:50, 11 December 2016
E. coli positive feedback cusR producer (short promoter)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 53
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 53
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 53
Illegal AgeI site found at 243 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed a genetic construct where the first half consisted of the cusR inducible promoter, a strong ribosome binding site (RBS) listed in the Parts Registry (BBa_B0035) and thecusR gene sourced from NCBI.