Difference between revisions of "Part:BBa M50051:Design"

 
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===References===
 
===References===
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1. Xavier KB and Bassler BL. 2005. Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli. J Bacteriol. 187(1): 238-248.

Revision as of 23:17, 11 December 2016


IPTG Inducible Plasmid for the Expression of LsrR Repressor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1134
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1134
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1134
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1134
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We did not run into any major design considerations after analyzing our construct using Gene Designer.


Source

The genetic sequence of the LsrR gene was sourced through the UniProt database. Other parts were sourced through DNA 2.0 under the plasmid name pD444-CH.

References

1. Xavier KB and Bassler BL. 2005. Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli. J Bacteriol. 187(1): 238-248.