Difference between revisions of "Part:BBa M50004:Experience"
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<I>Stanford BioE44 "Team WERK" 12.10.2016</I> | <I>Stanford BioE44 "Team WERK" 12.10.2016</I> | ||
<BR>We transformed E. coli with both BBa_M50004 and BBa_M50005 in an attempt to replicate the first step of A. borkumensis' alkane degradation in different bacteria. Under proper induction (IPTG), our Western blot protocol revealed moderate levels of alkB1 production. However, pH testing failed to confirm the cooperative function of alkB1 and alkG, with transformed bacteria in alkane-rich conditions behaving relatively indistinguishably from those in standard conditions. This may have been due to a failure in double transformation, with the two proteins being expressed at disproportionate levels. <BR><BR> | <BR>We transformed E. coli with both BBa_M50004 and BBa_M50005 in an attempt to replicate the first step of A. borkumensis' alkane degradation in different bacteria. Under proper induction (IPTG), our Western blot protocol revealed moderate levels of alkB1 production. However, pH testing failed to confirm the cooperative function of alkB1 and alkG, with transformed bacteria in alkane-rich conditions behaving relatively indistinguishably from those in standard conditions. This may have been due to a failure in double transformation, with the two proteins being expressed at disproportionate levels. <BR><BR> | ||
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Revision as of 22:05, 11 December 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_M50004
BBa_M50004 contains the alkB1 protein (ABO_2707) originating in the bacterium Alcanivorax borkumensis. It is meant to be used in conjunction with BBa_M50005 (alkG protein, ABO_2708) and other enzymes from A. borkumensis to degrade alkanes measuring up to 32 carbons as part of A. borkumensis' digestive process.
User Reviews
Stanford BioE44 "Team WERK" 12.10.2016
We transformed E. coli with both BBa_M50004 and BBa_M50005 in an attempt to replicate the first step of A. borkumensis' alkane degradation in different bacteria. Under proper induction (IPTG), our Western blot protocol revealed moderate levels of alkB1 production. However, pH testing failed to confirm the cooperative function of alkB1 and alkG, with transformed bacteria in alkane-rich conditions behaving relatively indistinguishably from those in standard conditions. This may have been due to a failure in double transformation, with the two proteins being expressed at disproportionate levels.
UNIQddd4cfa0325af8c2-partinfo-00000000-QINU UNIQddd4cfa0325af8c2-partinfo-00000001-QINU
Stanford Location
Plasmid name: ABO-2707
DNA2.0 Gene #: pD444-CF
Organism: E. coli
Device type: Actuator
Glycerol stock barcode: 0133027153
Box label: BioE44 F16